Researchers use CRISPR to store a movie in living bacteria

13 July, 2017, 01:02 | Author: Becky Perry
  • Researchers use CRISPR to store a movie in living bacteria

The bacteria E. coli might sometimes make us sick, but they have served as a workhorse in science.

Research at UC Berkeley and elsewhere suggests that CRISPR-Cas9 constantly feints with the cell's DNA fix system: as the enzyme cuts at its target site, the cell repairs the DNA, and CRISPR-Cas9 cuts again, repeating this vicious cycle until a mutation arises in the DNA that prevents enzyme binding, at which point the CRISPR-Cas9 molecule moves on to find another binding site. Now E. coli can claim one more skill: They can store our digital data.

Alan Davidson, a molecular geneticist at the University of Toronto who did not take part in the work, but is also collaborating with some of the study's authors, points out that two very similar papers were recently published in Nature this April and Molecular Cell in June.

Researchers are continually developing more efficient ways to store digital data.

RG: How much information could potentially be stored on DNA? Storing information in living DNA is more hard because the cells are always changing.

The technical achievement, reported on July 12 in Nature, is a step towards creating cellular recording systems that are capable of encoding a series of events, says Seth Shipman, a synthetic biologist at Harvard Medical School in Boston, Massachusetts. Researchers have used the microbial immune system CRISPR-Cas to encode a movie into the genome of the bacterium Escherichia coli.

They delivered the GIF into the living bacteria in the form of five frames: images of a galloping horse and rider, taken by English photographer Eadweard Muybridge, who produced the first stop-motion photographs in the 1870s. They reconstructed the image perfectly, and the video with 90 percent accuracy.

So it can be done. Stock our refrigerators with petri dishes full of family memories?

These anti-CRISPR proteins are likely to be a useful genetics, and perhaps clinical, tool. If we can use DNA to store information, we could also use it to record information, meaning we can use CRISPR to turn cells into tiny records of what they're doing and what's happening to them. That way we could flexibly create the DNA, avoiding problematic sequences.

Researchers use CRISPR to store a movie in living bacteria
Researchers use CRISPR to store a movie in living bacteria

"We want to use neurons to record a molecular history of the brain through development", Shipman said.

Before any of that happens, however, "there's a lot of important efficacy and safety [investigations] that still need to be done", Corn says. Humans start out as one power-packed ball of pluripotent stem cells, which can turn into anything: brain cells, organ-specific cells, blood cells. We care about that because we want to make cells that can record information from biological systems and store that information so that we can collect data without having to disrupt the biological system.

But first, Shipman needed to test the system with electronic data.

CRISPR (you say it like "crisper") stands for Clustered Regularly Interspaced Short Palindromic Repeats. CRISPR's job is to grab a piece of DNA from viral invaders and file it in the CRISPR section of its genome, generating a chronological record of invaders.

"We want to turn cells into historians", explained neuroscientist Seth Shipman, Ph.D., a post-doctoral fellow working with Church, in a statement. Sontheimer was not involved in the study, but he is a cofounder of Intellia Therapeutics, alongside study coauthor Jennifer Doudna of the University of California, Berkeley, and the company is using an anti-CRISPR protein that Sontheimer co-discovered (not AcrIIA4).

Jacob Corn, one of the first authors on the study, said: 'The ability to turn Cas9 gene editing off is just as important as the ability to turn it on.

CRISPR makes it easy to edit existing DNA or add new DNA anywhere in the genome.

Being able to then tap into cells' internal data recorders could become an invaluable tool for scientists.

RG: How is this possible with the CRISPR system? The researchers converted the individual pixels of each image into nucleotides, the building blocks of DNA.

China offers to mediate to resolve Kashmir issue
Gend said that the 1890 Sikkim-Tibet treaty confirmed the border, but India has been insisting on the contrary. How do you handle it is a test of our maturity.


  • Tony Hawk Pro Skater HD will be removed from Steam next week

    Tony Hawk Pro Skater HD will be removed from Steam next week

    If you want to nab the game before it's gone then you'll be pleased to learn it has seen an 80% discount and only costs a mere $8. The post explains that Tony Hawk's Pro Skater HD will be "retired from the store" on July 17.
    Vetr Inc. Upgrades Tesla Inc

    Vetr Inc. Upgrades Tesla Inc

    Their average price target spell out an upbeat performance - a -7% and would give TSLA a market capitalization of almost $50.98B. The electric vehicle producer reported ($1.97) EPS for the quarter, missing analysts' consensus estimates of ($0.67) by $1.30.
    Honduras vs French Guiana CONCACAF Gold Cup

    Honduras vs French Guiana CONCACAF Gold Cup

    He has accepted a great pass from Scott Arfield before unleashing a powerful shot that beat goalkeeper Patrick Pemberton. French Guiana have to get off to a better start if they want to advance to the quarterfinals in their Gold Cup debut.
  • The Deckers Outdoor Corporation (NYSE:DECK) Upgraded to Hold at BidaskClub

    Thirteen investment analysts have rated the stock with a hold rating and six have assigned a buy rating to the company's stock. KeyBanc Capital Markets initiated Applied Industrial Technologies (NYSE:AIT) on Tuesday, July 21 with "Sector Weight" rating.

    $0.23 EPS Expected for Potash Corporation of Saskatchewan Inc (POT)

    The Company, through its subsidiaries, is engaged in the generation, transmission, distribution and sale of electricity in SC . The stock's short float is around of 1.37% and short ratio is 2.15. 59 funds opened positions while 148 raised stakes.

    Aaron Rodgers Believes He's 'Starting The Back Nine' Of His Career

    As long as Rodgers is at his peak behind center, the Packers should always be considered contenders for the Lombardi Trophy. However, Jennings was speaking about the season after the Packers won the Super Bowl-when they won their first 13 games.
  • Congressmen file Article of Impeachment against President Trump

    An intermediary for the lawyer promised damaging information from the Russian government about Democrat Hillary Clinton. The filing of an impeachment resolution against a president in itself is not terribly uncommon.
    Woman arrested twice in same day after asking police to return drugs

    Woman arrested twice in same day after asking police to return drugs

    While returning to the station from the parking lot, Feole said he saw Morin get into the driver's seat of a blue Volvo S60. An officer confirmed that her license and registration was still suspended, then ran after Morin, ordering her to stop.

    Shares in McDonald's Corporation (NYSE:MCD) Acquired by GFS Advisors LLC

    The institutional investor owned 13,036 shares of the fast-food giant's stock after selling 793 shares during the period. YUM!" The stock of McDonald's Corporation (NYSE:MCD) earned "Outperform" rating by Credit Suisse on Monday, June 27.
  • Former SuperSport United defender Michael Boxall signs for Minnesota United

    Former SuperSport United defender Michael Boxall signs for Minnesota United

    Six seasons ago, Boxall was selected first by Vancouver in the 2011 MLS Supplemental Draft before the Whitecaps' inaugural season. Taking into account the MLS SuperDraft, Boxall was was the 55th overall selection that year.

    Zynga Inc. (ZNGA) Director Sells $43680.00 in Stock

    The transaction was disclosed in a legal filing with the Securities & Exchange Commission, which is available at this hyperlink . Bank of America Corporation raised their price objective on Zynga from $2.50 to $3.25 in a research note on Monday, May 15th.
    Chris Hemsworth Reacts to Brother Liam's Tiny Shorts: 'Was That Intentional?'

    Chris Hemsworth Reacts to Brother Liam's Tiny Shorts: 'Was That Intentional?'

    That felt like a late Friday night, drunken kinda post, ' Chris described as he held back laughter. Hemsworth told ET that the iconic brand approached him for the campaign more than six months ago.


Kroenke's Rams double in value after move to LA — Forbes report
Similarly, the Raiders do not receive a share of their relocation fee, but do get a share of the Rams' and Chargers fees. Rovell noted that each of the teams' relocation fees are based on the projected increase in franchise value.

Amitabh Bachchan sent legal notice to Kumar Vishwas
Reacting to the reports of legal notice, Vishwas tweeted that he was deleting the song from YouTube. Most notably, the Holi song Rang Barse from Amitabh Bachchan's Silsila contains lyrics by the poet.

Jason Bateman Breaks Bad in Netflix's Dark 'Ozark' Trailer
He serves as an executive producer on the show, and also directed four episodes. In fact, things have turned deadly serious for him.

Intrepid Potash, Inc (NYSE:IPI) Sees Significant Drop in Short Interest
The basic materials company reported ($0.15) EPS for the quarter, missing the consensus estimate of ($0.09) by $0.06. Regardless, closing prices are a useful tool that investors use to quantify changes in stock prices over time.

Harley-Davidson, Inc. (HOG) Upgraded at ValuEngine
Also, it has an estimated price-earnings (P/E) multiple of 12.54 and a trailing 12-month price-earnings (P/E) multiple of 14.85. The stock of Reliance Steel & Aluminum Co (NYSE:RS) has "Neutral" rating given on Tuesday, November 29 by Macquarie Research. International Ltd.
Bank Of Montreal /can/ reduced its holdings by selling 19,690 shares a decrease of 8.6% from 03/31/2017 to 06/30/2017. Sit Investment Associates Inc. raised its position in shares of worldwide by 13.8% in the first quarter.

'Dance Moms' star Abby Lee Miller reports to prison Wednesday
The website reported that dinner will be bean soup with a potato salad and either a hummus wrap or a deli sandwich. Wednesday was check-in day for the TV personality at the federal penitentiary in Victorville, Calif.

Free McDonald's ice cream for life? It could happen
The actual odds of winning depend on how many people redeem their free ice cream cone offer at the selected McDonald's . Anyone with the McDonald's mobile app can get a free vanilla cone on Sunday at participating McDonald's restaurants...

Rowe Price Group, Inc. (TROW) Position Increased by Banque Pictet & Cie SA
Many hedge fund investors lately purchased and unloaded stocks of TROW. 127 funds opened positions while 440 raised stakes. The Firm provides global investment management services through its subsidiaries to investors across the world.

Buffalo Bills defensive tackle arrested on weapons charge
The officer is pissed that Washington would put everyone in that situation and says, "I'm not trying to shoot anyone out here". The incident occurred around 10 p.m. local time Sunday night while police were clearing out a parking lot at Splash Park.